Kọlụm Ghost-Sniper Chromasir HPLC UPLC na-ewepụ ọnụ ọgụgụ mmụọ

nkowa nkenke:

Kọlụm Ghost-Sniper bụ ngwa ọrụ dị ike iji kpochapụ ọnụ ọgụgụ mmụọ emepụtara n'oge usoro nkewa chromatographic, ọkachasị na ọnọdụ gradient.Ọnụ elu mmụọ ga-ebute nsogbu ọnụọgụgụ ma ọ bụrụ na mmụọ elu kpuchiri elu nke mmasị.Site na kọlụm ghost-sniper Chromasir, enwere ike idozi ihe ịma aka niile sitere na ọnụ ọgụgụ mmụọ yana ọnụ ahịa oriri nnwale nwere ike belata nke ukwuu.

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Nkọwa ngwaahịa

Mkpado ngwaahịa

A na-arụpụta kọlụm mmụọ-sniper kpọmkwem iji kpochapụ ọnụ ọnụ mmụọ.Ọnụ elu mmụọ bụ nke amaghi ama na chromatogram, nke a na-ahụkarị n'oge usoro nkewa chromatographic na elution gradient ma ọ bụ ọrụ ogologo oge.Ihe omume nke elu mmụọ nwere mmetụta miri emi na nnwale ndị nyocha.Kọlụm Ghost-sniper na-eme iji dozie nsogbu ndị ahụ ma mee ka usoro nnwale dịkwuo adaba na nke ọma.Enwere ike itinye kọlụm a n'ọnọdụ dị oke egwu ma gosipụta mmetụta dị egwu.O doro anya na ọ bụ ụzọ dị mma isi wepụ nnyonye anya site na ọnụ ọgụgụ mmụọ na nkwenye usoro na nyocha ihe.

Oke

Akụkụ Mba.	Akụkụ	Olu	Ngwa
MC5046091P	50 × 4.6mm	ihe dịka 800ul	HPLC
MC3546092P	35 × 4.6mm	ihe dịka 580ul	HPLC
MC5021093P	50 × 2.1mm	ihe dịka 170ul	UPLC
MC3040096P	30 × 4.0mm	ihe dịka 380ul	HPLC Obere kọlụm

Nwụnye

Ngwa na nsonaazụ

Mkpachapụ anya

1. Ọ bụrụ na a na-ahazi nyocha batch na usoro HPLC, a na-atụ aro ka ịbawanye oge nguzozi maka ihe dịka 5 min - 10 min na ọnọdụ chromatographic gị, maka mmetụta nke olu nke kọlụm ghost-sniper.
2. Maka ogidi ọhụrụ, kpochaa ha na 100% acetonitrile na ọsọ ọsọ nke 0.5ml / min maka awa 4 tupu ejiri ya.
3. Ion-pair reagents na mobile na-adọ, nwere ike na-etinye obi gị dum site ghost-sniper kọlụm, nke nwere ike imetụta oge njigide na ọnụ ọgụgụ kasị elu udi nke gị lekwasịrị anya.Biko jiri nlezianya mee ihe n'okpuru usoro mkpanaka.
4. Oge ndụ kọlụm na-adabere na ọnọdụ nyocha, dị ka usoro mkpanaka.ihe mgbaze dị ọcha, na mmetọ nke akụrụngwa.Biko dochie kọlụm ghost-sniper mgbe niile iji hụ na arụmọrụ.
5. A na-atụ aro iji dochie kọlụm ghost-sniper ma ọ bụrụ na iwepụta mmetụta na-akawanye njọ ma ọ bụ enweghị ike imeju ihe achọrọ.
6. Dị ka akụkụ dị ọcha na chromatograph mmiri mmiri, kọlụm ghost-sniper nwere ike nyochaa ihe siri ike ma wepụ ihe ndị na-emerụ emerụ tupu injector.Kọlụm mmụọ-sniper na-enyekwa ngwá ọrụ na kọlụm chromatographic nwere nchebe ka mma, ma na-emezi chromatogram.
7. Ọ bụrụ na usoro mkpanaka nwere nnu nkpuchi, tupu na mgbe ejiri ya, nyefee ya na ngwọta 10% organic (10% methanol ma ọ bụ acetonitrile), iji zere nnu na-ekpuchi ma gbochie kọlụm.
8. Biko mara na ọ bụghị ọnụ ọgụgụ mmụọ niile nwere ike ijide kọlụm Ghost-Sniper.
9. Ọ bụrụ na kọlụm ahụ na-ahapụ nke a na-ejighị ya ogologo oge, a na-atụ aro ka idobe ya na ngwọta mmiri nke organic (70% methanol ma ọ bụ acetonitrile).Ma biko kpochaa ya na 100% acetonitrile na ọnụego erute nke 0.5 ml / min maka 1 awa tupu iji ya.