Kọlụm Ghost-Sniper Chromasir HPLC UPLC na-ewepụ ọnụ ọgụgụ mmụọ
A na-arụpụta kọlụm mmụọ-sniper kpọmkwem iji kpochapụ ọnụ ọnụ mmụọ. Ọnụ ọgụgụ mmụọ bụ nke amaghi ama na chromatogram, nke a na-ahụkarị n'oge usoro nkewa nke chromatographic na gradient elution ma ọ bụ ọrụ ogologo oge. Ihe omume nke elu mmụọ nwere mmetụta miri emi na nnwale ndị nyocha. Kọlụm Ghost-sniper na-eme iji dozie nsogbu ndị ahụ ma mee ka usoro nnwale dịkwuo adaba na nke ọma. Enwere ike itinye kọlụm a n'ọnọdụ dị oke egwu ma gosipụta mmetụta dị egwu. O doro anya na ọ bụ ụzọ dị mma isi wepụ nnyonye anya site na ọnụ ọgụgụ mmụọ na nkwenye usoro na nyocha ihe.
| Akụkụ Mba. | Akụkụ | Olu | Ngwa |
| MC5046091P | 50 × 4.6mm | ihe dịka 800ul | HPLC |
| MC3546092P | 35 × 4.6mm | ihe dịka 580ul | HPLC |
| MC5021093P | 50 × 2.1mm | ihe dịka 170ul | UPLC |
| MC3040096P | 30 × 4.0mm | ihe dịka 380ul | HPLC Obere kọlụm |
Nwụnye
Ngwa na nsonaazụ
1. Ọ bụrụ na a na-ahazi nyocha batch na usoro HPLC, a na-atụ aro ka ịbawanye oge nguzozi maka ihe dịka 5 min - 10 min na ọnọdụ chromatographic gị, maka mmetụta nke olu nke kọlụm ghost-sniper.
2. Maka ogidi ọhụrụ, kpochaa ha na 100% acetonitrile na ọsọ ọsọ nke 0.5ml / min maka awa 4 tupu ejiri ya.
3. Ion-pair reagents na mobile na-adọ, nwere ike na-etinye obi gị dum site ghost-sniper kọlụm, nke nwere ike imetụta oge njigide na ọnụ ọgụgụ kasị elu udi nke gị lekwasịrị anya. Biko jiri nlezianya mee ihe n'okpuru usoro mkpanaka.
4. Oge ndụ kọlụm na-adabere na ọnọdụ nyocha, dị ka usoro mkpanaka. ihe mgbaze dị ọcha, na mmetọ nke akụrụngwa. Biko dochie kọlụm ghost-sniper mgbe niile iji hụ na arụmọrụ.
5. A na-atụ aro iji dochie kọlụm ghost-sniper ma ọ bụrụ na iwepụta mmetụta na-akawanye njọ ma ọ bụ enweghị ike imeju ihe achọrọ.
6. Dị ka akụkụ dị ọcha na chromatograph mmiri mmiri, kọlụm ghost-sniper nwere ike nyochaa ihe ndị siri ike ma wepụ ihe ndị na-emerụ emerụ tupu injector. Kọlụm mmụọ-sniper na-enyekwa ngwá ọrụ na kọlụm chromatographic nwere nchebe ka mma, ma na-emezi chromatogram.
7. Ọ bụrụ na usoro mkpanaka nwere nnu nkpuchi, tupu na mgbe ejiri ya, nyefee ya na ngwọta nke 10% organic (10% methanol ma ọ bụ acetonitrile), iji zere nnu na-ekpuchi ma gbochie kọlụm.
8. Biko mara na ọ bụghị ọnụ ọgụgụ mmụọ niile nwere ike ijide kọlụm Ghost-Sniper.
9. Ọ bụrụ na kọlụm ahụ na-ahapụ nke a na-ejighị ya ogologo oge, a na-atụ aro ka idobe ya na ngwọta mmiri nke organic (70% methanol ma ọ bụ acetonitrile). Ma biko kpochaa ya na 100% acetonitrile na ọnụego erute nke 0.5 ml / min maka 1 awa tupu iji ya.
